Mini Review Acetaminophen Toxicity and 5 Oxoproline (Pyroglutamic Acid): A Tale of Two Cycles, One an ATP Depleting Futile Cycle Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione, Alcohol and drugs Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification Acetaminophen at low doses depletes airway glutathione and alters respiratory reflex responses Smith 2013 The FASEB Journal Wiley Online Library